Basic Principles in Flow Cytometry - Print

This book introduces readers to the principles of flow cytometry and flow cytometer operation, as well as cell proliferation, labeling, enumeration, analysis, and apoptosis/cell death.

Flow cytometry was initially used in the diagnosis and treatment of malignant diseases. Its use has broadened to include other areas, such as fetomaternal hemorrhage, immunodeficiencies, paroxysmal nocturnal hemoglobinuria, cellular therapy, and genetic variation to name but a few. Unlike other laboratory devices, flow cytometers present the technologist with many different settings for their use. This book is intended as a primer to help the novice understand the basic principles of this evolving technology and provide a foundation for further study.

Topics addressed:

• Overview
• Fluorescence, Dyes, and Fluorescence Compensation
• DNA Analysis
• Data Analysis
• Cell Proliferation
• Cell Death and Apoptosis
• Applications in Clinical Practice
• Quality Control

(AABB Press, 2013, soft cover, 91 pages)

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