Practical Handbook of Cellular Therapy Cryopreservation

Long-term storage of cryopreserved hematopoietic cells and their recovery after thawing are of critical importance in both traditional and novel cellular therapies.

The increasing use of traditional hematopoietic cell therapies—as well as the emerging potential of novel regenerative medicine therapies—highlights the importance of the cryopreservation process and its impact on cell yield, viability, and function. This Practical Handbook of Cellular Cryopreservation explains the “nuts and bolts” of the cryopreservation process from beginning to end: which freezers are used for which products, the fundamentals of freezing cells without damage, how to read freezing curves, how to handle products after an abnormality occurs, when to validate/verify/qualify, and so much more. This volume is packed with color illustrations that bring additional clarity to these detailed discussions.

Topics include:

• Cellular cryopreservation overview
• Comparison of cryopreservation systems
• Freezing curves and their interpretation
• Preventing cryoinjury
• Product characterization after thawing
• Transient warming events and other abnormalities
• Compliance with accreditation standards

Written by Michael H. Creer, MD; Aby J. Mathew, PhD; and M. Victor Lemas, PhD

(AABB Press, 2015, soft cover, color illus., 157 pages, ISBN 978-1-56395-869-4)

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